Equipment

PicoQuant MicroTime 200

Fluorescence lifetime imaging microscope Fluorescence correlation spectroscopy Single-molecule sensitivity Feasibility of combined FLIM / AFM measurement FLIM, FCS, FLCS, FRET inverted microscope body XYZ objective-scanning by piezo-stage possibility of independent-scanning AFM on top of the stage – JPK NanoWizard 3 pulsed laser excitation: wavelength 5313 nm, spectral width < 1 nm, 2 mW @ 40 MHz three-channel detection: 2x single-photon avalanche diode, fiber-coupled spectrograph Shamrock 303i/Andor iXon EMCCD picosecond event timer and Time-Correlated-Single Photon Counting (TCSPC) module HydraHarp 400 objectives: 10x dry, 40x water immersion, 100x oil immersion

Horiba FluoroMAX4

Scientific-grade fluorimeter TCSPC – Time-resolved fluorescence Plate-reader option Polarization/Anisotropy option scanning excitation and emission single-grating monochromators (Czerny-Turner type) CW, 150 W Ozone-free xenon arc lamp R928P photon counting PMT (185-850 nm) for emission detection reference photodiode detector variable monochromator bandwidth 0 – 30 nm variable integration time 1 ms – 60 s motorized Glan-Thompson polarizers for fluorescence polarization/anisotropy measurements fiber-coupled accessory plate reader Horiba MicroMax 384 NanoLED pulsed laser/LED driver for time-resolved measurements several laser/LED heads for pulsed excitation: 375, 405, 470, 635 nm Horiba DeltaHub for Time-Correlated-Single Photon Counting (TCSPC) measurements with sub-ns temporal resolution temperature control of sample

Laboratory soft X-ray source

Laser-produced plasma (incoherent) Extended applications Nd:YAG 1064nm/800mJ/7ns laser for generation of plasma SXR wavelength range approx. 1 - 20 nm (depending on the target gas) SXR pulse length ~5ns (Ar target) brilliance ~ 1.7 x 1013 photon/pulse/sr (Ar target) capacious vacuum chamber with computer-driven positioning system variety of electrical, mechanical, and optical feedthroughs

Shimadzu UV-3600

UV-VIS-NIR absorption spectrophotometer Thermoelectrical temperature controller Integration sphere accessory detection range 185 – 3300 nm dual-beam double dual-grating monochromator, ultra-low stray-light level (0.00005 % max. at 340 nm) three detectors: photomultiplier (UV-VIS), InGaAs, and PbS (near IR) integration sphere attachment with PMT, InGaAs, and PbS detectors for transmission or reflectance measurement variable monochromator bandwidth variable scanning speed thermoelectrical sample temperature control holder for cuvettes with up-to 100 mm path length holder for solid-state plate samples

Carl Zeiss ConfoCor2

Fluorescence (lifetime) correlation spectroscopy Custom TCSPC upgrade fiber-coupled Ar laser for continuous-wave excitation (458, 488, 514 nm) custom-upgraded to allow time-resolved FCS PicoQuant TimeHarp 200 TCSPC board PicoQuant PDL 800-B picosecond Pulsed Diode Laser Driver pulsed laser heads 470 and 640 nm